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Opis strony Advanced BLAST
(proszę korzystać z oryginalnej strony, bo poniżej jest tylko atrapa)


[Message of the day]
    Choose program to use and database to search:

    ProgramDatabase
 

The query sequence is filtered for low complexity regions by default.

Depending on server load your search may take several seconds to several minutes.
Note: Nothing will be returned until your search is complete.

Enter here your input data as 
 

Please read about FASTA format description

Advanced options for the BLAST server:

Expect Cutoff Matrix
Strand Filter   Histogram   NCBI-gi
Descriptions Alignments

Other advanced options: 

The BLAST server may be very busy during the weekday, resulting in delays for users. The email option allows a user to receive the results quickly in a convenient form. If the HTML option is used, the results should be loaded into a web browser for viewing.

   Send reply to the Email address:    In HTML format

 

 

 Comments and suggestions to: < blast-help@ncbi.nlm.nih.gov >
Credits to: Sergei B. Shavirin
Acknowledgements to: Tom Madden and Jonathan Epstein

To discover any relatives your sequence may have in the database, use the left-hand mouse button to retrieve part of the sequence (say ~60 residues) and paste into the Query sequence box of the BLAST  at NCBI facility. Edit out  any non-amino acid characters from the pasted sequence and Perform Search - this should take around 30 seconds to run, but the server may be busy with other jobs, so please be patient. When the search is complete, make a note of high-scoring matches - you may wish to save the result using Save as in Netscape's File option (top left-hand corner of the border).

Uwagi.

1. blastx (przeszukiwanie bazy sekwencji białkowych sekwencją nukleotydową) daje bardziej wiarygodne wyniki niż blastn.

2. Proszę nie używać BLAST do szukania homologów polipeptydów (<10aa) i oligonucleotydów. Do szukania krótkimi sekwencjami lepiej nadaje się FASTA.

4. Aby zachować wyniki BLAST należy w Netscape otworzyć stronę z wynikiem w  Composer (EDIT PAGE) i po (repost form data) zachować (save as) w oddzielnym klatalogu na dysku lokalnym.
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